ABSTRACT
Objective@#To investigate the changes of oxidative stress in kidney of type 2 diabetic rats after sleeve gastrectomy and its effect on renal function.@*Methods@#Twenty male SD rats were fed with high-fat diet and injected with streptozotocin intraperitoneally to induce type 2 diabetes mellitus. Seventeen successful induction models were divided into sham operation group (n=7) and sleeve gastrectomy (SG) group (n=10) by random number table method, and were treated with sham operation and SG respectively. The body weight, 24-hour food intake, fasting blood glucose (FBG)and serum glucagon-like peptide-1 (GLP-1) were measured before and 1, 2, 3 and 4 weeks after operation. The activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and malondialdehyde in renal tissue as well as blood urea nitrogen (BUN), creatinine levels and 24-hour urinary microalbumin (UALB) were measured 4 weeks after operation. Masurement data conforming to normal distribution were expressed as Mean±SD, and t-test was used for comparison between two groups.@*Results@#At the 1st, 2nd, 3rd, 4th weeks after operation, the levels of FBG in SG group was lower than those in sham operation group [(11.13±3.27) mmol/L vs (16.74±4.10) mmol/L, (9.53±2.82) mmol/L vs (19.31±3.66) mmol/L, (6.69±2.44) mmol/L vs (20.84±2.71) mmol/L, (6.58±2.96) mmol/L vs (19.99±2.85) mmol/L, all P<0.05]. In the same period, the levels of serum GLP-1 in SG group was higher than those in sham operation group [(22.61±2.92) pg/mL vs (15.42±2.39) pg/mL, (24.72±3.02) pg/mL vs (16.20±2.26) pg/mL, (24.59±2.85) pg/mL vs (15.84±2.75) pg/mL, (26.15±3.23) pg/mL vs (15.77±2.79) pg/mL, all P<0.05]. At the 4th week after operation, BUN[(5.34±0.82) mmol/L], creatinine[(39.78±6.13)]μmol/L, UALB[(31.18±6.88) mg/24 h] and renal creatinine[(5.84±0.85) nmol/mg] in SG group were lower than those in sham operation group [BUN (9.08±1.54) mmol/L, creatinine (64.07±8.63) μmol/L, UALB (67.64±9.07) mg/24 h, creatinine (10.78±1.28) nmol/mg], and the differences were statistically significant (all P<0.05). Renal SOD[(620.05±55.98) U/mg], CAT[(24.72±2.28) U/mg] and GSH-Px[(281.53±27.99) U] in SG Group were lower than those in sham operation group [SOD(392.52±45.97) U/mg, catalase (15.62±2.46) U/mg, GSH-Px (164.71±21.83) U], and the differences were statistically significant (all P<0.05).@*Conclusion@#Sleeve gastrectomy may improve renal function through reducing oxidative stress in the kidney.
ABSTRACT
Objective To investigate the changes of oxidative stress in kidney of type 2 diabetic rats after sleeve gastrectomy and its effect on renal function.Methods Twenty male SD rats were fed with high-fat diet and injected with streptozotocin intraperitoneally to induce type 2 diabetes mellitus.Seventeen successful induction models were divided into sham operation group (n =7) and sleeve gastrectomy (SG) group (n =10) by random number table method,and were treated with sham operation and SG respectively.The body weight,24-hour food intake,fasting blood glucose (FBG)and serum glucagon-like peptide-1 (GLP-1) were measured before and 1,2,3 and 4 weeks after operation.The activities of superoxide dismutase (SOD),catalase,glutathione peroxidase (GSH-Px) and malondialdehyde in renal tissue as well as blood urea nitrogen (BUN),creatinine levels and 24-hour urinary microalbumin (UALB) were measured 4 weeks after operation.Masurement data conforming to normal distribution were expressed as Mean ± SD,and t-test was used for comparison between two groups.Results At the 1st,2nd,3rd,4th weeks after operation,the levels of FBG in SG group was lower than those in sham operation group [(11.13 ±3.27) mmol/L vs (16.74 ±4.10) mmol/L,(9.53 ±2.82) mmol/L vs (19.31 ±3.66) mmol/ L,(6.69 ±2.44) mmol/L vs (20.84 ±2.71) mmol/L,(6.58 ±2.96) mmol/L vs (19.99 ±2.85) mmol/L,all P ＜ 0.05].In the same period,the levels of serum GLP-1 in SG group was higher than those in sham operation group [(22.61 ± 2.92) pg/mL vs (15.42 ± 2.39) pg/mL,(24.72 ± 3.02) pg/mL vs (16.20 ± 2.26) pg/mL,(24.59 ±2.85) pg/mL vs (15.84±2.75) pg/mL,(26.15 ±3.23) pg/mL vs (15.77 ±2.79) pg/mL,all P＜ 0.05].At the 4th week after operation,BUN[(5.34 ±0.82) mmol/L],creatinine[(39.78 ±6.13)] μmoL/L,UALB [(31.18 ± 6.88) mg/24 h] and renal creatinine [(5.84 ± 0.85) nmol/mg] in SG group were lower than those in sham operation group [BUN (9.08 ± 1.54) mmol/L,creatinine (64.07 ± 8.63) μmol/L,UALB (67.64 ±9.07) mg/24 h,creatinine (10.78 ± 1.28) nmol/mg],and the differences were statistically significant (all P＜0.05).Renal SOD[(620.05 ±55.98) U/mg],CAT[(24.72 ±2.28) U/mg] and GSH-Px[(281.53 ± 27.99) U] in SG Group were lower than those in sham operation group [SOD(392.52 ± 45.97) U/mg,catalase (15.62 ± 2.46) U/mg,GSH-Px (164.71 ± 21.83) U],and the differences were statistically significant (all P ＜ 0.05).Conclusion Sleeve gastrectomy may improve renal function through reducing oxidative stress in the kidney.